precast polyacrylamide gels Search Results


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anamed Elektrophorese GmbH pre-cast gradient 4–20% tris-glycine gels
Pre Cast Gradient 4–20% Tris Glycine Gels, supplied by anamed Elektrophorese GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioExpress pre-cast polyacrylamide gels
Pre Cast Polyacrylamide Gels, supplied by BioExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre-cast polyacrylamide gels - by Bioz Stars, 2026-09
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Gel Company Inc 2d hpe large gels
2d Hpe Large Gels, supplied by Gel Company Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation 10% surepage polyacrylamide gel
10% Surepage Polyacrylamide Gel, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation 15% precast mini polyacrylamide gels genscript, nanjing, china
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
15% Precast Mini Polyacrylamide Gels Genscript, Nanjing, China, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
15% precast mini polyacrylamide gels genscript, nanjing, china - by Bioz Stars, 2026-09
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GenScript corporation native polyacrylamide gels
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
Native Polyacrylamide Gels, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
native polyacrylamide gels - by Bioz Stars, 2026-09
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SERVA Electrophoresis 4–20% precast polyacrylamide gels 43277.01
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
4–20% Precast Polyacrylamide Gels 43277.01, supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
4–20% precast polyacrylamide gels 43277.01 - by Bioz Stars, 2026-09
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BioExpress 10% tris-hcl polyacrylamide precast gels
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
10% Tris Hcl Polyacrylamide Precast Gels, supplied by BioExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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CBS Scientific precast polyacrylamide gels (4–30)
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
Precast Polyacrylamide Gels (4–30), supplied by CBS Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
precast polyacrylamide gels (4–30) - by Bioz Stars, 2026-09
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PEQLAB 12% sodium dodecyl sulfate-polyacrylamide gel nuview precast gels
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
12% Sodium Dodecyl Sulfate Polyacrylamide Gel Nuview Precast Gels, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
12% sodium dodecyl sulfate-polyacrylamide gel nuview precast gels - by Bioz Stars, 2026-09
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GenScript corporation 10% polyacrylamide 12 or 15-well precast gels
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
10% Polyacrylamide 12 Or 15 Well Precast Gels, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10% polyacrylamide 12 or 15-well precast gels - by Bioz Stars, 2026-09
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ATTO Corp precast tricine-sds 15% polyacrylamide gels e-pagel e-t/r15s
Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini <t>polyacrylamide</t> gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
Precast Tricine Sds 15% Polyacrylamide Gels E Pagel E T/R15s, supplied by ATTO Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini polyacrylamide gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.

Journal: International Journal of Molecular Sciences

Article Title: De Novo Computational Design of a Lipase with Hydrolysis Activity towards Middle-Chained Fatty Acid Esters

doi: 10.3390/ijms24108581

Figure Lengend Snippet: Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini polyacrylamide gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.

Article Snippet: All the reagents were commercially available and used as received: isopropyl β-D-1-thiogalactopyranoside (Solarbio, Beijing, China); kanamycin (Solarbio, Beijing, China); p-nitrophenyl octanoate (Sigma-Aldrich, St. Louis, MO, USA); plasmid mini kit I (Omega, Norcross, GA, USA); gel DNA extraction mini kit (Vazyme, Nanjing, China); Bradford protein assay kit (Tiangen, Beijing, China); 15% precast mini polyacrylamide gels (Genscript, Nanjing, China); and BeyoGoldTM His-tag Purification Resin (Beyotime, Shanghai, China).

Techniques: SDS Page, Molecular Weight, Activity Assay

Experimental data of the redesigned enzymes. ( a ) SDS-PAGE of the redesigned 1a8uD 1 using 15% precast mini polyacrylamide gel. Lane M: standard protein molecular weight; lane 1: wt–1a8u; lane 2: 1a8uD 1 ; lane 3: 1a8uD 1 –M1; lane 4: 1a8uD 1 –M2; lane 5: 1a8uD 1 –M3; lane 6: 1a8uD 1 –M4; lane 7: 1a8uD 1 –M5; lane 8: 1a8uD 1 –M6; lane 9: 1a8uD 1 –M7; lane 10: 1a8uD 1 –M8; lane 11: 1a8uD 1 –M9. ( b ) Qualitative analysis of redesigned enzymes according to color change. The holes with active enzymes, and their surrounding area, turned yellow.

Journal: International Journal of Molecular Sciences

Article Title: De Novo Computational Design of a Lipase with Hydrolysis Activity towards Middle-Chained Fatty Acid Esters

doi: 10.3390/ijms24108581

Figure Lengend Snippet: Experimental data of the redesigned enzymes. ( a ) SDS-PAGE of the redesigned 1a8uD 1 using 15% precast mini polyacrylamide gel. Lane M: standard protein molecular weight; lane 1: wt–1a8u; lane 2: 1a8uD 1 ; lane 3: 1a8uD 1 –M1; lane 4: 1a8uD 1 –M2; lane 5: 1a8uD 1 –M3; lane 6: 1a8uD 1 –M4; lane 7: 1a8uD 1 –M5; lane 8: 1a8uD 1 –M6; lane 9: 1a8uD 1 –M7; lane 10: 1a8uD 1 –M8; lane 11: 1a8uD 1 –M9. ( b ) Qualitative analysis of redesigned enzymes according to color change. The holes with active enzymes, and their surrounding area, turned yellow.

Article Snippet: All the reagents were commercially available and used as received: isopropyl β-D-1-thiogalactopyranoside (Solarbio, Beijing, China); kanamycin (Solarbio, Beijing, China); p-nitrophenyl octanoate (Sigma-Aldrich, St. Louis, MO, USA); plasmid mini kit I (Omega, Norcross, GA, USA); gel DNA extraction mini kit (Vazyme, Nanjing, China); Bradford protein assay kit (Tiangen, Beijing, China); 15% precast mini polyacrylamide gels (Genscript, Nanjing, China); and BeyoGoldTM His-tag Purification Resin (Beyotime, Shanghai, China).

Techniques: SDS Page, Molecular Weight