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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: De Novo Computational Design of a Lipase with Hydrolysis Activity towards Middle-Chained Fatty Acid Esters
doi: 10.3390/ijms24108581
Figure Lengend Snippet: Experimental data of de novo designed enzymes. ( a ) SDS-PAGE of de novo designed enzymes using 15% precast mini polyacrylamide gel. Lane M: standard protein molecular weight (180/140/100/75/60/45/35/25/15/10 kDa); lane 1: supernatant of 1a8uD 1 ; lane 2: inclusion bodies of 1a8uD 1 ; lane 3: supernatant of 1jbwD 1 ; lane 4: inclusion bodies of 1jbwD 1 ; lane 5: supernatant of 1zowD 1 ; lane 6: inclusion bodies of 1zowD 1 ; lane 7: supernatant of 1jdyD 1 ; lane 8: inclusion bodies of 1jdyD 1 . ( b ) Qualitative analysis of designed enzymes based on colorimetric activity assay. The lysates of enzymes were separately added into the corresponding hole in an agarose Petri dish. The area with active designs turned yellow, while the area containing the blank solution and inactive proteins displayed no color change.
Article Snippet: All the reagents were commercially available and used as received: isopropyl β-D-1-thiogalactopyranoside (Solarbio, Beijing, China); kanamycin (Solarbio, Beijing, China); p-nitrophenyl octanoate (Sigma-Aldrich, St. Louis, MO, USA); plasmid mini kit I (Omega, Norcross, GA, USA); gel DNA extraction mini kit (Vazyme, Nanjing, China); Bradford protein assay kit (Tiangen, Beijing, China); 15%
Techniques: SDS Page, Molecular Weight, Activity Assay
Journal: International Journal of Molecular Sciences
Article Title: De Novo Computational Design of a Lipase with Hydrolysis Activity towards Middle-Chained Fatty Acid Esters
doi: 10.3390/ijms24108581
Figure Lengend Snippet: Experimental data of the redesigned enzymes. ( a ) SDS-PAGE of the redesigned 1a8uD 1 using 15% precast mini polyacrylamide gel. Lane M: standard protein molecular weight; lane 1: wt–1a8u; lane 2: 1a8uD 1 ; lane 3: 1a8uD 1 –M1; lane 4: 1a8uD 1 –M2; lane 5: 1a8uD 1 –M3; lane 6: 1a8uD 1 –M4; lane 7: 1a8uD 1 –M5; lane 8: 1a8uD 1 –M6; lane 9: 1a8uD 1 –M7; lane 10: 1a8uD 1 –M8; lane 11: 1a8uD 1 –M9. ( b ) Qualitative analysis of redesigned enzymes according to color change. The holes with active enzymes, and their surrounding area, turned yellow.
Article Snippet: All the reagents were commercially available and used as received: isopropyl β-D-1-thiogalactopyranoside (Solarbio, Beijing, China); kanamycin (Solarbio, Beijing, China); p-nitrophenyl octanoate (Sigma-Aldrich, St. Louis, MO, USA); plasmid mini kit I (Omega, Norcross, GA, USA); gel DNA extraction mini kit (Vazyme, Nanjing, China); Bradford protein assay kit (Tiangen, Beijing, China); 15%
Techniques: SDS Page, Molecular Weight